Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes

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Summary: High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological diegojavierfares.com and therapeutic discoveries.High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling.Here, we describe a protocol for epitope and RNA-preserving dissociation of adult mouse brains and subsequent leukocyte purification and staining, which is adaptable to homeostatic and pathogenic brains.Leukocyte preparation following click here this protocol permits exquisite single-cell surface protein and RNA profiling in applications including CyTOF and CITE-seq.For complete details on the use and execution of this protocol, please refer to Guldner et al.

(2020) and Golomb et al.(2020).