Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes
Summary: High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological diegojavierfares.com and therapeutic discoveries.High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling.Here, we describe a protocol for epitope and RNA-prese